Phyloseq.Rmd processing Data_Karoline_16S_2025

author: ""
date: '`r format(Sys.time(), "%d %m %Y")`'
header-includes:
   - \usepackage{color, fancyvrb}
output:
  rmdformats::readthedown:
    highlight: kate
    number_sections : yes    
  pdf_document: 
    toc: yes
    toc_depth: 2
    number_sections : yes
---

```{r, echo=FALSE, warning=FALSE}
## Global options
# TODO: reproduce the html with the additional figure/SVN-files for editing.
# IMPORTANT NOTE: needs before "mkdir figures"
#NEEDs to be often close R and start R, then new rendering --> working!
#rmarkdown::render('Phyloseq.Rmd',output_file='Phyloseq.html')
#install.packages("heatmaply")
#install.packages("gplots")
#BiocManager::install("phyloseq")
#library(phyloseq)
#DEBUG a package conflict: using phyloseq::tax_table() or detach(package:MicrobiotaProcess, unload=TRUE)
```

```{r load-packages, include=FALSE}

#install.packages(c("picante", "rmdformats"))
#mamba install -c conda-forge freetype libpng harfbuzz fribidi
#mamba install -c conda-forge r-systemfonts r-svglite r-kableExtra freetype fontconfig harfbuzz fribidi libpng
library(knitr)
library(rmdformats)
library(readxl)
library(dplyr)
library(kableExtra)
library(openxlsx)
library(DESeq2)

options(max.print="75")
knitr::opts_chunk$set(fig.width=8, 
                      fig.height=6, 
                      eval=TRUE, 
                      cache=TRUE,
                      echo=TRUE,
                      prompt=FALSE,
                      tidy=FALSE,
                      comment=NA,
                      message=FALSE,
                      warning=FALSE)
opts_knit$set(width=85)
# Phyloseq R library
#* Phyloseq web site : https://joey711.github.io/phyloseq/index.html
#* See in particular tutorials for
#    - importing data: https://joey711.github.io/phyloseq/import-data.html
#    - heat maps: https://joey711.github.io/phyloseq/plot_heatmap-examples.html
```

# Data

Import raw data and assign sample key:

```{r, echo=FALSE, warning=FALSE}
#extend qiime2_metadata_for_qza_to_phyloseq.tsv with Diet and Flora
#setwd("~/DATA/Data_Laura_16S_2/core_diversity_e4753")
#map_corrected <- read.csv("qiime2_metadata_for_qza_to_phyloseq.tsv", sep="\t", row.names=1)
#knitr::kable(map_corrected) %>% kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))
```

# Prerequisites to be installed

* R : https://pbil.univ-lyon1.fr/CRAN/
* R studio : https://www.rstudio.com/products/rstudio/download/#download

```R
install.packages("dplyr")     # To manipulate dataframes
install.packages("readxl")    # To read Excel files into R
install.packages("ggplot2")   # for high quality graphics
install.packages("heatmaply")
source("https://bioconductor.org/biocLite.R")
biocLite("phyloseq")
```

```{r libraries, echo=TRUE, message=FALSE}
#mamba install -c conda-forge r-ggplot2 r-vegan r-data.table
#BiocManager::install("microbiome")
#install.packages("ggpubr")
#install.packages("heatmaply")
library("readxl") # necessary to import the data from Excel file
library("ggplot2") # graphics
library("picante")
library("microbiome") # data analysis and visualisation
library("phyloseq") # also the basis of data object. Data analysis and visualisation
library("ggpubr") # publication quality figures, based on ggplot2
library("dplyr") # data handling, filter and reformat data frames
library("RColorBrewer") # nice color options
library("heatmaply")
library(vegan)
library(gplots)
```

# Read the data and create phyloseq objects

Three tables are needed

* OTU
* Taxonomy
* Samples

```{r, echo=FALSE, warning=FALSE}

    library(tidyr)

    # For QIIME1
    #ps.ng.tax <- import_biom("./exported_table/feature-table.biom", "./exported-tree/tree.nwk")

    # For QIIME2
    #install.packages("remotes")
    #remotes::install_github("jbisanz/qiime2R")
    #"core_metrics_results/rarefied_table.qza", rarefying performed in the code, therefore import the raw table.
    library(qiime2R)
    ps.ng.tax <- qza_to_phyloseq(
      features =  "dada2_tests2/test_7_f240_r240/table.qza",
      tree = "rooted-tree.qza",
      metadata = "qiime2_metadata_for_qza_to_phyloseq.tsv"
    )
    # or
    #biom convert \
    #      -i ./exported_table/feature-table.biom \
    #      -o ./exported_table/feature-table-v1.biom \
    #      --to-json
    #ps.ng.tax <- import_biom("./exported_table/feature-table-v1.biom", treefilename="./exported-tree/tree.nwk")

    sample <- read.csv("./qiime2_metadata_for_qza_to_phyloseq.tsv", sep="\t", row.names=1)
    SAM = sample_data(sample, errorIfNULL = T)
    #rownames(SAM) <- c("1","2","3","5","6","7","8","9","10","12","13","14","15","16","17","18","19","20","21","22","23","24","25","26","27","28","29","30","31","32","33","34","35","36","37","38","39","40","41","42","43","44","46","47","48","49","50","51","52","53","55")

    #> setdiff(rownames(SAM), sample_names(ps.ng.tax))
    #[1] "sample-L9" should be removed since the low reads

    ps.ng.tax <- merge_phyloseq(ps.ng.tax, SAM)
    print(ps.ng.tax)

    taxonomy <- read.delim("exported-taxonomy/taxonomy.tsv", sep="\t", header=TRUE)
    #head(taxonomy)
    # Separate taxonomy string into separate ranks
    taxonomy_df <- taxonomy %>% separate(Taxon, into = c("Domain","Phylum","Class","Order","Family","Genus","Species"), sep = ";", fill = "right", extra = "drop")
    # Use Feature.ID as rownames
    rownames(taxonomy_df) <- taxonomy_df$Feature.ID
    taxonomy_df <- taxonomy_df[, -c(1, ncol(taxonomy_df))]  # Drop Feature.ID and Confidence
    # Create tax_table
    tax_table_final <- phyloseq::tax_table(as.matrix(taxonomy_df))
    # Merge tax_table with existing phyloseq object
    ps.ng.tax <- merge_phyloseq(ps.ng.tax, tax_table_final)
    # Check
    ps.ng.tax

    #colnames(phyloseq::tax_table(ps.ng.tax)) <- c("Domain","Phylum","Class","Order","Family","Genus","Species")
    saveRDS(ps.ng.tax, "./ps.ng.tax.rds")
```

Visualize data
```{r, echo=TRUE, warning=FALSE}
  sample_names(ps.ng.tax)
  rank_names(ps.ng.tax)
  sample_variables(ps.ng.tax)

  # Define sample names once
  samples <- c(
    "sample-A1","sample-A2","sample-A3","sample-A4","sample-A5","sample-A6","sample-A7","sample-A8","sample-A9","sample-A10","sample-A11",
    "sample-B1","sample-B2","sample-B3","sample-B4","sample-B5","sample-B6","sample-B7","sample-B8","sample-B9","sample-B10","sample-B11","sample-B12","sample-B13","sample-B14","sample-B15","sample-B16",
    "sample-C1","sample-C2","sample-C3","sample-C4","sample-C5","sample-C6","sample-C7","sample-C8","sample-C9","sample-C10",
    "sample-E1","sample-E2","sample-E3","sample-E4","sample-E5","sample-E6","sample-E7","sample-E8","sample-E9","sample-E10",
    "sample-F1","sample-F2","sample-F3","sample-F4","sample-F5",
    "sample-G1","sample-G2","sample-G3","sample-G4","sample-G5","sample-G6",
    "sample-H1","sample-H2","sample-H3","sample-H4","sample-H5","sample-H6",
    "sample-I1","sample-I2","sample-I3","sample-I4","sample-I5","sample-I6",
    "sample-J1","sample-J2","sample-J3","sample-J4","sample-J10","sample-J11",  #RESIZED: "sample-J5","sample-J6","sample-J7","sample-J8","sample-J9",
    "sample-K7","sample-K8","sample-K9","sample-K10",  #RESIZED: "sample-K1","sample-K2","sample-K3","sample-K4","sample-K5","sample-K6",  "sample-K11","sample-K12","sample-K13","sample-K14","sample-K15",
    "sample-L1","sample-L7","sample-L8","sample-L10",  #RESIZED: "sample-L2","sample-L3","sample-L4","sample-L5","sample-L6",  "sample-L11","sample-L12","sample-L13","sample-L14","sample-L15",
    "sample-M1","sample-M2","sample-M3","sample-M4","sample-M5","sample-M6","sample-M7","sample-M8",
    "sample-N1","sample-N2","sample-N3","sample-N4","sample-N5","sample-N6","sample-N7","sample-N8","sample-N9","sample-N10",
    "sample-O1","sample-O2","sample-O3","sample-O4","sample-O5","sample-O6","sample-O7","sample-O8"
  )
  ps.ng.tax <- prune_samples(samples, ps.ng.tax)

  sample_names(ps.ng.tax)
  rank_names(ps.ng.tax)
  sample_variables(ps.ng.tax)
```


Normalize number of reads in each sample using median sequencing depth.
```{r, echo=TRUE, warning=FALSE}
# RAREFACTION
set.seed(9242)  # This will help in reproducing the filtering and nomalisation.
ps.ng.tax <- rarefy_even_depth(ps.ng.tax, sample.size = 6389)
total <- 6389

# NORMALIZE number of reads in each sample using median sequencing depth.
total = median(sample_sums(ps.ng.tax))
#> total
#[1] 42369
standf = function(x, t=total) round(t * (x / sum(x)))
ps.ng.tax = transform_sample_counts(ps.ng.tax, standf)
ps.ng.tax_rel <- microbiome::transform(ps.ng.tax, "compositional")

saveRDS(ps.ng.tax, "./ps.ng.tax.rds")
hmp.meta <- meta(ps.ng.tax)
hmp.meta$sam_name <- rownames(hmp.meta)
```




# Prepare ps.ng.tax_rel, ps.ng.tax_abund, ps.ng.tax_abund_rel from ps.ng.tax

```{r, echo=FALSE, warning=FALSE}
#MOVE_FROM_ABOVE: The number of reads used for normalization is **`r sprintf("%.0f", total)`**. 
#A basic heatmap using the default parameters.
#  plot_heatmap(ps.ng.tax, method = "NMDS", distance = "bray")
#NOTE that giving the correct OTU numbers in the text (1%, 0.5%, ...)!!!
```

For the heatmaps, we focus on the most abundant OTUs by first converting counts to relative abundances within each sample. We then filter to retain only OTUs whose mean relative abundance across all samples exceeds 0.1% (0.001). We are left with 199 OTUs which makes the reading much more easy.

```{r, echo=FALSE, warning=FALSE}

# Custom function to plot a heatmap with the specified sample order
#plot_heatmap_custom <- function(ps, sample_order, method = "NMDS", distance = "bray") {

# --Filtering strategy 1: This filters taxa based on raw counts (ps.ng.tax). For each taxon (across samples), it checks if it has a count that exceeds 1% of the total in at least one sample.     Description: We consider the most abundant OTUs for heatmaps. For example one can only take OTUs that represent at least 1% of reads in at least one sample. Remember we normalized all the sampples to median number of reads (total).  We are left with only 382 OTUS which makes the reading much more easy.
#ps.ng.tax_abund <- phyloseq::filter_taxa(ps.ng.tax, function(x) sum(x > total*0.01) > 0, TRUE)

# --Filtering strategy 2: This filters taxa based on relative abundances (ps.ng.tax_rel). It keeps only those taxa whose mean relative abundance across samples exceeds 0.1%.
# 1) Convert to relative abundances
ps.ng.tax_rel <- transform_sample_counts(ps.ng.tax, function(x) x / sum(x))

# 2) Get the logical vector of which OTUs to keep (based on relative abundance)
keep_vector <- phyloseq::filter_taxa(
  ps.ng.tax_rel,
  function(x) mean(x) > 0.001,
  prune = FALSE
)

# 3) Use the TRUE/FALSE vector to subset absolute abundance data
ps.ng.tax_abund <- prune_taxa(names(keep_vector)[keep_vector], ps.ng.tax)

# 4) Normalize the final subset to relative abundances per sample
ps.ng.tax_abund_rel <- transform_sample_counts(
  ps.ng.tax_abund,
  function(x) x / sum(x)
)
```






# Heatmaps


```{r, echo=FALSE, warning=FALSE}
datamat_ = as.data.frame(otu_table(ps.ng.tax_abund))

#datamat <- datamat_[c("1","2","5","6","7",  "8","9","10","12","13","14",    "15","16","17","18","19","20",  "21","22","23","24","25","26","27","28",    "29","30","31","32",  "33","34","35","36","37","38","39","51",    "40","41","42","43","44","46",  "47","48","49","50","52","53","55")]
datamat <- datamat_[c(
    "sample-A1","sample-A2","sample-A3","sample-A4","sample-A5","sample-A6","sample-A7","sample-A8","sample-A9","sample-A10","sample-A11",
    "sample-B1","sample-B2","sample-B3","sample-B4","sample-B5","sample-B6","sample-B7","sample-B8","sample-B9","sample-B10","sample-B11","sample-B12","sample-B13","sample-B14","sample-B15","sample-B16",
    "sample-C1","sample-C2","sample-C3","sample-C4","sample-C5","sample-C6","sample-C7","sample-C8","sample-C9","sample-C10",
    "sample-E1","sample-E2","sample-E3","sample-E4","sample-E5","sample-E6","sample-E7","sample-E8","sample-E9","sample-E10",
    "sample-F1","sample-F2","sample-F3","sample-F4","sample-F5",
    "sample-G1","sample-G2","sample-G3","sample-G4","sample-G5","sample-G6",
    "sample-H1","sample-H2","sample-H3","sample-H4","sample-H5","sample-H6",
    "sample-I1","sample-I2","sample-I3","sample-I4","sample-I5","sample-I6",
    "sample-J1","sample-J2","sample-J3","sample-J4","sample-J10","sample-J11",  #RESIZED: "sample-J5","sample-J6","sample-J7","sample-J8","sample-J9",
    "sample-K7","sample-K8","sample-K9","sample-K10",  #RESIZED: "sample-K1","sample-K2","sample-K3","sample-K4","sample-K5","sample-K6",  "sample-K11","sample-K12","sample-K13","sample-K14","sample-K15",
    "sample-L1","sample-L7","sample-L8","sample-L10",  #RESIZED: "sample-L2","sample-L3","sample-L4","sample-L5","sample-L6",  "sample-L11","sample-L12","sample-L13","sample-L14","sample-L15",
    "sample-M1","sample-M2","sample-M3","sample-M4","sample-M5","sample-M6","sample-M7","sample-M8",
    "sample-N1","sample-N2","sample-N3","sample-N4","sample-N5","sample-N6","sample-N7","sample-N8","sample-N9","sample-N10",
    "sample-O1","sample-O2","sample-O3","sample-O4","sample-O5","sample-O6","sample-O7","sample-O8"
  )]

hr <- hclust(as.dist(1-cor(t(datamat), method="pearson")), method="complete")
hc <- hclust(as.dist(1-cor(datamat, method="spearman")), method="complete")
mycl = cutree(hr, h=max(hr$height)/1.08)
mycol = c("YELLOW", "DARKBLUE", "DARKORANGE", "DARKMAGENTA", "DARKCYAN", "DARKRED",  "MAROON", "DARKGREEN", "LIGHTBLUE", "PINK", "MAGENTA", "LIGHTCYAN","LIGHTGREEN", "BLUE", "ORANGE", "CYAN", "RED", "GREEN");

mycol = mycol[as.vector(mycl)]
sampleCols <- rep('GREY',ncol(datamat))
#names(sampleCols) <- c("Group1", "Group1", "Group1", "Group1", "Group1",   "Group2", "Group2",   "Group3", "Group3", "Group3",  ...)

# Define 14 colors
my_colors <- c("#a6cee3", "#1f78b4", "#b2df8a", "#33a02c",
                "#fb9a99", "#e31a1c", "#fdbf6f", "#ff7f00",
                "#cab2d6", "#6a3d9a", "#ffff99", "#b15928",
                "#8dd3c7", "#fb8072")
# Example column names
colnames(datamat) <- c(
    "sample-A1","sample-A2","sample-A3","sample-A4","sample-A5","sample-A6","sample-A7","sample-A8","sample-A9","sample-A10","sample-A11",
    "sample-B1","sample-B2","sample-B3","sample-B4","sample-B5","sample-B6","sample-B7","sample-B8","sample-B9","sample-B10","sample-B11","sample-B12","sample-B13","sample-B14","sample-B15","sample-B16",
    "sample-C1","sample-C2","sample-C3","sample-C4","sample-C5","sample-C6","sample-C7","sample-C8","sample-C9","sample-C10",
    "sample-E1","sample-E2","sample-E3","sample-E4","sample-E5","sample-E6","sample-E7","sample-E8","sample-E9","sample-E10",
    "sample-F1","sample-F2","sample-F3","sample-F4","sample-F5",
    "sample-G1","sample-G2","sample-G3","sample-G4","sample-G5","sample-G6",
    "sample-H1","sample-H2","sample-H3","sample-H4","sample-H5","sample-H6",
    "sample-I1","sample-I2","sample-I3","sample-I4","sample-I5","sample-I6",
    "sample-J1","sample-J2","sample-J3","sample-J4","sample-J10","sample-J11",  #RESIZED: "sample-J5","sample-J6","sample-J7","sample-J8","sample-J9",
    "sample-K7","sample-K8","sample-K9","sample-K10",  #RESIZED: "sample-K1","sample-K2","sample-K3","sample-K4","sample-K5","sample-K6",  "sample-K11","sample-K12","sample-K13","sample-K14","sample-K15",
    "sample-L1","sample-L7","sample-L8","sample-L10",  #RESIZED: "sample-L2","sample-L3","sample-L4","sample-L5","sample-L6",  "sample-L11","sample-L12","sample-L13","sample-L14","sample-L15",
    "sample-M1","sample-M2","sample-M3","sample-M4","sample-M5","sample-M6","sample-M7","sample-M8",
    "sample-N1","sample-N2","sample-N3","sample-N4","sample-N5","sample-N6","sample-N7","sample-N8","sample-N9","sample-N10",
    "sample-O1","sample-O2","sample-O3","sample-O4","sample-O5","sample-O6","sample-O7","sample-O8"
  )
# (replace with your actual column names)

# Remove "sample-" prefix for easier matching
sample_names <- sub("^sample-", "", colnames(datamat))

# Create a function to match sample IDs to groups
assign_group <- function(sample_id) {
  # First letter indicates group
  prefix <- substr(sample_id, 1, 1)
  switch(prefix,
         "A" = 1,
         "B" = 2,
         "C" = 3,
         "E" = 4,
         "F" = 5,
         "G" = 6,
         "H" = 7,
         "I" = 8,
         "J" = 9,
         "K" = 10,
         "L" = 11,
         "M" = 12,
         "N" = 13,
         "O" = 14,
         NA)
}
# Assign group numbers to samples
group_numbers <- sapply(sample_names, assign_group)
# Assign colors based on group numbers
sampleCols <- my_colors[group_numbers]
# Check results
print(sampleCols)
#'#a6cee3', '#1f78b4', '#b2df8a', '#33a02c', '#fb9a99', '#e31a1c', '#cab2d6', '#6a3d9a'

#bluered(75)
#color_pattern <- colorRampPalette(c("blue", "white", "red"))(100)
library(RColorBrewer)
custom_palette <- colorRampPalette(brewer.pal(9, "Blues"))
heatmap_colors <- custom_palette(100)
#colors <- heatmap_color_default(100)
png("figures/heatmap.png", width=1200, height=2400)
#par(mar=c(2, 2, 2, 2))  , lwid=1    lhei=c(0.7, 10)) # Adjust height of color keys   keysize=0.3,
heatmap.2(as.matrix(datamat),Rowv=as.dendrogram(hr),Colv = NA, dendrogram = 'row',
            scale='row',trace='none',col=heatmap_colors, cexRow=1.2, cexCol=1.5,
            RowSideColors = mycol, ColSideColors = sampleCols, srtCol=15, labRow=row.names(datamat), key=TRUE, margins=c(10, 15), lhei=c(0.7, 15), lwid=c(1,8))
dev.off()
```
```{r, echo=TRUE, warning=FALSE, fig.cap="Heatmap", out.width = '100%', fig.align= "center"}
knitr::include_graphics("./figures/heatmap.png")
```

\pagebreak





```{r, echo=FALSE, warning=FALSE}
  library(stringr)
#FITTING1: 
#for id in 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100  101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199; do
#for id in 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300; do
#for id in 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382; do
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Domain\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Domain\"], \"__\")[[1]][2]"
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Phylum\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Phylum\"], \"__\")[[1]][2]"
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Class\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Class\"], \"__\")[[1]][2]"
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Order\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Order\"], \"__\")[[1]][2]"
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Family\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Family\"], \"__\")[[1]][2]"
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Genus\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Genus\"], \"__\")[[1]][2]"
#  echo "phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Species\"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[${id},\"Species\"], \"__\")[[1]][2]"
#done

phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[1,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[2,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[3,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[4,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[5,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[6,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[7,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[8,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[9,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[10,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[11,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[12,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[13,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[14,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[15,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[16,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[17,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[18,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[19,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[20,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[21,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[22,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[23,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[24,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[25,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[26,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[27,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[28,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[29,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[30,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[31,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[32,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[33,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[34,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[35,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[36,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[37,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[38,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[39,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[40,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[41,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[42,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[43,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[44,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[45,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[46,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[47,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[48,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[49,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[50,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[51,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[52,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[53,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[54,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[55,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[56,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[57,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[58,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[59,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[60,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[61,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[62,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[63,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[64,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[65,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[66,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[67,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[68,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[69,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[70,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[71,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[72,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[73,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[74,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[75,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[76,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[77,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[78,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[79,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[80,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[81,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[82,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[83,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[84,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[85,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[86,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[87,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[88,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[89,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[90,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[91,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[92,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[93,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[94,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[95,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[96,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[97,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[98,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[99,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[100,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[101,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[102,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[103,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[104,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[105,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[106,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[107,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[108,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[109,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[110,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[111,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[112,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[113,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[114,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[115,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[116,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[117,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[118,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[119,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[120,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[121,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[122,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[123,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[124,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[125,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[126,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[127,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[128,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[129,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[130,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[131,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[132,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[133,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[134,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[135,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[136,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[137,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[138,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[139,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[140,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[141,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[142,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[143,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[144,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[145,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[146,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[147,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[148,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[149,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[150,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[151,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[152,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[153,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[154,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[155,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[156,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[157,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[158,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[159,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[160,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[161,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[162,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[163,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[164,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[165,"Species"], "__")[[1]][2]

phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[166,"Species"], "__")[[1]][2]

phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Species"], "__")[[1]][2]

phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[167,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[168,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[169,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[170,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[171,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[172,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[173,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[174,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[175,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[176,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[177,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[178,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[179,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[180,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[181,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[182,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[183,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[184,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[185,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[186,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[187,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[188,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[189,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[190,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[191,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[192,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[193,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[194,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[195,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[196,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[197,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[198,"Species"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Domain"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Domain"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Phylum"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Phylum"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Class"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Class"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Order"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Order"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Family"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Family"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Genus"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Genus"], "__")[[1]][2]
phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Species"] <- str_split(phyloseq::tax_table(ps.ng.tax_abund_rel)[199,"Species"], "__")[[1]][2]

```

# Taxonomic summary

## Bar plots in phylum level

```{r, fig.width=16, fig.height=8, echo=TRUE, warning=FALSE}
  #aes(color="Phylum", fill="Phylum") --> aes()
  #ggplot(data=data, aes(x=Sample, y=Abundance, fill=Phylum)) 
  my_colors <- c("darkblue", "darkgoldenrod1", "darkseagreen", "darkorchid", "darkolivegreen1", "lightskyblue", "darkgreen", "deeppink", "khaki2", "firebrick", "brown1", "darkorange1", "cyan1", "royalblue4", "darksalmon", "darkblue","royalblue4", "dodgerblue3", "steelblue1", "lightskyblue", "darkseagreen", "darkgoldenrod1", "darkseagreen", "darkorchid", "darkolivegreen1", "brown1", "darkorange1", "cyan1", "darkgrey")
  plot_bar(ps.ng.tax_abund_rel, fill="Phylum") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black")) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=2))                                  #6 instead of theme.size
```
```{r, echo=FALSE, warning=FALSE}
  #png("abc.png")
  #knitr::include_graphics("./Phyloseq_files/figure-html/unnamed-chunk-7-1.png")
  #dev.off()
```


\pagebreak
Regroup together pre vs post stroke samples and normalize number of reads in each group using median sequencing depth.

```{r, echo=TRUE, warning=FALSE}
  ps.ng.tax_abund_rel_pre_post_stroke <- merge_samples(ps.ng.tax_abund_rel, "pre_post_stroke")
  #PENDING: The effect weighted twice by sum(x), is the same to the effect weighted once directly from absolute abundance?!
  ps.ng.tax_abund_rel_pre_post_stroke_ = transform_sample_counts(ps.ng.tax_abund_rel_pre_post_stroke, function(x) x / sum(x))
  #plot_bar(ps.ng.tax_abund_relSampleType_, fill = "Phylum") + geom_bar(aes(color=Phylum, fill=Phylum), stat="identity", position="stack")
  plot_bar(ps.ng.tax_abund_rel_pre_post_stroke_, fill="Phylum") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"))
```


```{r, echo=FALSE, warning=FALSE}

  #FITTING6: regulate the bar height if it has replicates: 11+16+10+10+5+6+6+6+11+15+14+8+10+8=136


  ps.ng.tax_abund_rel_weighted <- data.table::copy(ps.ng.tax_abund_rel)

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A1")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A10")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A11")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A11")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A2")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A3")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A4")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A5")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A6")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A7")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A8")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-A9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-A9")]/11

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B1")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B10")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B11")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B11")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B12")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B12")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B13")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B13")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B14")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B14")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B15")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B15")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B16")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B16")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B2")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B3")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B4")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B5")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B6")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B7")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B8")]/16
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-B9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-B9")]/16

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C1")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C10")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C2")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C3")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C4")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C5")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C6")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C7")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C8")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-C9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-C9")]/10

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E1")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E10")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E2")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E3")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E4")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E5")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E6")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E7")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E8")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-E9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-E9")]/10

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-F1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-F1")]/5
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-F2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-F2")]/5
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-F3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-F3")]/5
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-F4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-F4")]/5
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-F5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-F5")]/5

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-G1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-G1")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-G2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-G2")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-G3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-G3")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-G4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-G4")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-G5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-G5")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-G6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-G6")]/6

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-H1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-H1")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-H2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-H2")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-H3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-H3")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-H4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-H4")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-H5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-H5")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-H6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-H6")]/6

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-I1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-I1")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-I2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-I2")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-I3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-I3")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-I4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-I4")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-I5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-I5")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-I6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-I6")]/6

  #RESIZED:
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J1")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J2")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J3")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J4")]/6
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J5")]/11
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J6")]/11
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J7")]/11
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J8")]/11
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J9")]/11
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J10")]/6
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-J11")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-J11")]/6

  #RESIZED:
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K1")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K2")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K3")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K4")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K5")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K6")]/15
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K7")]/4
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K8")]/4
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K9")]/4
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K10")]/4
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K11")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K11")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K12")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K12")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K13")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K13")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K14")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K14")]/15
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-K15")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-K15")]/15

  #RESIZED:
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L1")]/4
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L2")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L3")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L4")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L5")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L6")]/14
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L7")]/4
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L8")]/4
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L10")]/4
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L11")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L11")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L12")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L12")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L13")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L13")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L14")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L14")]/14
  #otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-L15")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-L15")]/14

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M1")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M2")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M3")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M4")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M5")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M6")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M7")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-M8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-M8")]/8

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N1")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N10")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N10")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N2")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N3")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N4")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N5")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N6")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N7")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N8")]/10
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-N9")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-N9")]/10

  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O1")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O1")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O2")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O2")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O3")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O3")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O4")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O4")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O5")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O5")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O6")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O6")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O7")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O7")]/8
  otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O8")] <- otu_table(ps.ng.tax_abund_rel)[,c("sample-O8")]/8

  sum(otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O1")])
  #[1] 0.125
  sum(otu_table(ps.ng.tax_abund_rel)[,c("sample-O1")])
  #[1] 1
```

\pagebreak
Use color according to phylum. Do separate panels Stroke and Sex_age.

```{r, echo=FALSE, warning=FALSE}
  #plot_bar(ps.ng.tax_abund_relswab_, x="Phylum", fill = "Phylum", facet_grid = Patient~RoundDay) + geom_bar(aes(color=Phylum, fill=Phylum), stat="identity", position="stack") + theme(axis.text = element_text(size = theme.size, colour="black"))
  plot_bar(ps.ng.tax_abund_rel_weighted, x="Phylum", fill="Phylum", facet_grid = pre_post_stroke~Sex_age) + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"), axis.text.x=element_blank(), axis.ticks=element_blank()) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=2))
```







## Bar plots in class level

```{r, fig.width=16, fig.height=8, echo=TRUE, warning=FALSE}
  my_colors <- c("darkblue", "darkgoldenrod1", "darkseagreen", "darkorchid", "darkolivegreen1", "lightskyblue", "darkgreen", "deeppink", "khaki2", "firebrick", "brown1", "darkorange1", "cyan1", "royalblue4", "darksalmon", "darkblue","royalblue4", "dodgerblue3", "steelblue1", "lightskyblue", "darkseagreen", "darkgoldenrod1", "darkseagreen", "darkorchid", "darkolivegreen1", "brown1", "darkorange1", "cyan1", "darkgrey")
  plot_bar(ps.ng.tax_abund_rel, fill="Class") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black")) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=3))
```

Regroup together pre vs post stroke samples and normalize number of reads in each group using median sequencing depth.
```{r, echo=TRUE, warning=FALSE}
  plot_bar(ps.ng.tax_abund_rel_pre_post_stroke_, fill="Class") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"))
```
\pagebreak


Use color according to class. Do separate panels Stroke and Sex_age.
```{r, echo=TRUE, warning=FALSE}
  #NOTE: MANALLY RUNNING the CODE by COPYING the CODE under R-console for the 6 blocks, then show them with knitr::include_graphics
  sum(otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O1")])
  plot_bar(ps.ng.tax_abund_rel_weighted, x="Class", fill="Class", facet_grid = pre_post_stroke~Sex_age) + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"), axis.text.x=element_blank(), axis.ticks=element_blank()) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=3))
```

```{r, echo=FALSE, warning=FALSE}
        ps_df <- phyloseq::psmelt(ps.ng.tax_abund_rel_weighted)
        # 准备数据
        ps_summary <- ps_df %>%
          # 1. 只保留这三个 condition
          filter(pre_post_stroke %in% c("pre.antibiotics", "baseline", "pre.stroke")) %>%

          # 2. 聚合
          group_by(Sex_age, pre_post_stroke, Class) %>%
          summarise(Abundance = sum(Abundance), .groups = "drop") %>%

          # 3. 设置 factor 顺序和重命名
          mutate(
            # 替换 Sex_age 名称
            Sex_age = recode(Sex_age,
                            "female.aged" = "Female (Aged)",
                            "male.aged"   = "Male (Aged)",
                            "male.young"  = "Male (Young)"),
            Sex_age = factor(Sex_age, levels = c("Male (Aged)", "Female (Aged)", "Male (Young)")),

            # 替换 condition 名称
            pre_post_stroke = recode(pre_post_stroke,
                                    "pre.antibiotics" = "Before Antibiotics",
                                    "baseline"        = "Baseline",
                                    "pre.stroke"      = "Before Stroke"),
            pre_post_stroke = factor(pre_post_stroke,
                                    levels = c("Before Antibiotics", "Baseline", "Before Stroke")),

            Class = factor(Class)
          )

        # 确保颜色数匹配
        class_levels <- levels(ps_summary$Class)
        color_map <- setNames(my_colors[seq_along(class_levels)], class_levels)

        # 绘图
        p <- ggplot(ps_summary, aes(x = Sex_age, y = Abundance, fill = Class)) +
          geom_bar(stat = "identity", position = "stack", width = 0.55) +  # 更窄的柱子
          facet_grid(pre_post_stroke ~ ., scales = "free_x", drop = TRUE) +
          scale_fill_manual(values = color_map, drop = FALSE) +
          theme_minimal(base_size = 11) +
          theme(
            axis.text.x = element_text(angle = 45, hjust = 1, size = 9, colour = "black"),
            axis.title = element_text(size = 11),
            strip.text = element_text(size = 10, face = "bold"),
            legend.position = "right",               # ✅ legend 放右边
            legend.title = element_blank(),
            panel.grid.major.x = element_blank(),
            panel.grid.minor = element_blank()
          ) +
          guides(fill = guide_legend(ncol = 1)) +     # 竖排图例
          labs(
            x = "Sex and Age Group",
            y = "Relative Abundance",
            title = "Taxonomic Class Composition by Group and Condition"
          )

        # 保存为 PNG 文件
        ggsave(
          filename = "./figures/Separate_Stroke_and_SexAge_on_Class.png",
          plot = p,
          width = 8,
          height = 6,
          dpi = 200
        )

        knitr::include_graphics("./figures/Separate_Stroke_and_SexAge_on_Class.png")
```


```{r, echo=FALSE, warning=FALSE}
        ps_df <- phyloseq::psmelt(ps.ng.tax_abund_rel_weighted)
        # 数据处理，只保留 "Before Stroke"
        ps_summary <- ps_df %>%
          filter(pre_post_stroke == "pre.stroke") %>%
          group_by(Sex_age, Class) %>%
          summarise(Abundance = sum(Abundance), .groups = "drop") %>%
          mutate(
            Sex_age = recode(Sex_age,
              "female.aged" = "Female (Aged)",
              "male.aged" = "Male (Aged)",
              "male.young" = "Male (Young)"
            ),
            Sex_age = factor(Sex_age, levels = c("Male (Aged)", "Female (Aged)", "Male (Young)")),
            Class = factor(Class)
          )

        # 映射颜色
        class_levels <- levels(ps_summary$Class)
        color_map <- setNames(my_colors[seq_along(class_levels)], class_levels)

        # 绘图
        p <- ggplot(ps_summary, aes(x = Sex_age, y = Abundance, fill = Class)) +
          geom_bar(stat = "identity", position = "stack", width = 0.55) +
          scale_fill_manual(values = color_map, drop = FALSE) +
          theme_minimal(base_size = 11) +
          theme(
            axis.text.x = element_text(angle = 45, hjust = 1, size = 9, colour = "black"),
            axis.title = element_text(size = 11),
            legend.position = "right",
            legend.title = element_blank(),
            panel.grid.major.x = element_blank(),
            panel.grid.minor = element_blank()
          ) +
          labs(
            x = "Sex and Age Group",
            y = "Relative Abundance",
            title = "Class Composition - Before Stroke"
          ) +
          guides(fill = guide_legend(ncol = 2))

        # 保存图像
        ggsave(
          filename = "./figures/Before_Stroke_Class_Composition.png",
          plot = p,
          width = 8,
          height = 5,
          dpi = 200
        )

        # 插入图像到报告
        knitr::include_graphics("./figures/Before_Stroke_Class_Composition.png")
```







## Bar plots in order level

```{r, fig.width=16, fig.height=8, echo=TRUE, warning=FALSE}
  my_colors <- c("darkblue", "darkgoldenrod1", "darkseagreen", "darkorchid", "darkolivegreen1", "lightskyblue", "darkgreen", "deeppink", "khaki2", "firebrick", "brown1", "darkorange1", "cyan1", "royalblue4", "darksalmon", "darkblue","royalblue4", "dodgerblue3", "steelblue1", "lightskyblue", "darkseagreen", "darkgoldenrod1", "darkseagreen", "darkorchid", "darkolivegreen1", "brown1", "darkorange1", "cyan1", "darkgrey")
  plot_bar(ps.ng.tax_abund_rel, fill="Order") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black")) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=4))
```

Regroup together pre vs post stroke and normalize number of reads in each group using median sequencing depth.
```{r, echo=TRUE, warning=FALSE}
  plot_bar(ps.ng.tax_abund_rel_pre_post_stroke_, fill="Order") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"))
```
\pagebreak

Use color according to order. Do separate panels Stroke and Sex_age.
```{r, echo=FALSE, warning=FALSE}

  #FITTING7: regulate the bar height if it has replicates
  sum(otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O1")])
  plot_bar(ps.ng.tax_abund_rel_weighted, x="Order", fill="Order", facet_grid = pre_post_stroke~Sex_age) + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"), axis.text.x=element_blank(), axis.ticks=element_blank()) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=4))
```

```{r, echo=FALSE, warning=FALSE}
        ps_df <- phyloseq::psmelt(ps.ng.tax_abund_rel_weighted)
        # 准备数据
        ps_summary <- ps_df %>%
          # 1. 只保留这三个 condition
          filter(pre_post_stroke %in% c("pre.antibiotics", "baseline", "pre.stroke")) %>%

          # 2. 聚合
          group_by(Sex_age, pre_post_stroke, Order) %>%
          summarise(Abundance = sum(Abundance), .groups = "drop") %>%

          # 3. 设置 factor 顺序和重命名
          mutate(
            # 替换 Sex_age 名称
            Sex_age = recode(Sex_age,
                            "female.aged" = "Female (Aged)",
                            "male.aged"   = "Male (Aged)",
                            "male.young"  = "Male (Young)"),
            Sex_age = factor(Sex_age, levels = c("Male (Aged)", "Female (Aged)", "Male (Young)")),

            # 替换 condition 名称
            pre_post_stroke = recode(pre_post_stroke,
                                    "pre.antibiotics" = "Before Antibiotics",
                                    "baseline"        = "Baseline",
                                    "pre.stroke"      = "Before Stroke"),
            pre_post_stroke = factor(pre_post_stroke,
                                    levels = c("Before Antibiotics", "Baseline", "Before Stroke")),

            Order = factor(Order)
          )

        # 确保颜色数匹配
        class_levels <- levels(ps_summary$Order)
        color_map <- setNames(my_colors[seq_along(class_levels)], class_levels)

        # 绘图
        p <- ggplot(ps_summary, aes(x = Sex_age, y = Abundance, fill = Order)) +
          geom_bar(stat = "identity", position = "stack", width = 0.55) +  # 更窄的柱子
          facet_grid(pre_post_stroke ~ ., scales = "free_x", drop = TRUE) +
          scale_fill_manual(values = color_map, drop = FALSE) +
          theme_minimal(base_size = 11) +
          theme(
            axis.text.x = element_text(angle = 45, hjust = 1, size = 9, colour = "black"),
            axis.title = element_text(size = 11),
            strip.text = element_text(size = 10, face = "bold"),
            legend.position = "right",               # ✅ legend 放右边
            legend.title = element_blank(),
            panel.grid.major.x = element_blank(),
            panel.grid.minor = element_blank()
          ) +
          guides(fill = guide_legend(ncol = 1)) +     # 竖排图例
          labs(
            x = "Sex and Age Group",
            y = "Relative Abundance",
            title = "Taxonomic Order Composition by Group and Condition"
          )

        # 保存为 PNG 文件
        ggsave(
          filename = "./figures/Separate_Stroke_and_SexAge_on_Order.png",
          plot = p,
          width = 8,
          height = 6,
          dpi = 200
        )

        knitr::include_graphics("./figures/Separate_Stroke_and_SexAge_on_Order.png")
```

```{r, echo=FALSE, warning=FALSE}
        ps_df <- phyloseq::psmelt(ps.ng.tax_abund_rel_weighted)
        # 数据处理，只保留 "Before Stroke"
        ps_summary <- ps_df %>%
          filter(pre_post_stroke == "pre.stroke") %>%
          group_by(Sex_age, Order) %>%
          summarise(Abundance = sum(Abundance), .groups = "drop") %>%
          mutate(
            Sex_age = recode(Sex_age,
              "female.aged" = "Female (Aged)",
              "male.aged" = "Male (Aged)",
              "male.young" = "Male (Young)"
            ),
            Sex_age = factor(Sex_age, levels = c("Male (Aged)", "Female (Aged)", "Male (Young)")),
            Order = factor(Order)
          )

        # 映射颜色
        class_levels <- levels(ps_summary$Order)
        color_map <- setNames(my_colors[seq_along(class_levels)], class_levels)

        # 绘图
        p <- ggplot(ps_summary, aes(x = Sex_age, y = Abundance, fill = Order)) +
          geom_bar(stat = "identity", position = "stack", width = 0.55) +
          scale_fill_manual(values = color_map, drop = FALSE) +
          theme_minimal(base_size = 11) +
          theme(
            axis.text.x = element_text(angle = 45, hjust = 1, size = 9, colour = "black"),
            axis.title = element_text(size = 11),
            legend.position = "right",
            legend.title = element_blank(),
            panel.grid.major.x = element_blank(),
            panel.grid.minor = element_blank()
          ) +
          labs(
            x = "Sex and Age Group",
            y = "Relative Abundance",
            title = "Order Composition - Before Stroke"
          ) +
          guides(fill = guide_legend(ncol = 2))

        # 保存图像
        ggsave(
          filename = "./figures/Before_Stroke_Order_Composition.png",
          plot = p,
          width = 8,
          height = 5,
          dpi = 200
        )

        # 插入图像到报告
        knitr::include_graphics("./figures/Before_Stroke_Order_Composition.png")
```








## Bar plots in family level

```{r, fig.width=16, fig.height=8, echo=TRUE, warning=FALSE}
  my_colors <- c(
          "#FF0000", "#000000", "#0000FF", "#C0C0C0", "#FFFFFF", "#FFFF00", "#00FFFF", "#FFA500", "#00FF00", "#808080", "#FF00FF", "#800080", "#FDD017", "#0000A0", "#3BB9FF", "#008000", "#800000", "#ADD8E6", "#F778A1", "#800517", "#736F6E", "#F52887", "#C11B17", "#5CB3FF", "#A52A2A", "#FF8040", "#2B60DE", "#736AFF", "#1589FF", "#98AFC7", "#8D38C9", "#307D7E", "#F6358A", "#151B54", "#6D7B8D", "#FDEEF4", "#FF0080", "#F88017", "#2554C7", "#FFF8C6", "#D4A017", "#306EFF", "#151B8D", "#9E7BFF", "#EAC117", "#E0FFFF", "#15317E", "#6C2DC7", "#FBB917", "#FCDFFF", "#15317E", "#254117", "#FAAFBE", "#357EC7"
        )
  plot_bar(ps.ng.tax_abund_rel, fill="Family") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black")) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=8))
```

Regroup together pre vs post stroke samples and normalize number of reads in each group using median sequencing depth.
```{r, echo=TRUE, warning=FALSE}
  plot_bar(ps.ng.tax_abund_rel_pre_post_stroke_, fill="Family") + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"))
```
\pagebreak

Use color according to family. Do separate panels Stroke and Sex_age.
```{r, echo=TRUE, warning=FALSE}
  sum(otu_table(ps.ng.tax_abund_rel_weighted)[,c("sample-O1")])
  plot_bar(ps.ng.tax_abund_rel_weighted, x="Family", fill="Family", facet_grid = pre_post_stroke~Sex_age) + geom_bar(aes(), stat="identity", position="stack") +
  scale_fill_manual(values = my_colors) + theme(axis.text = element_text(size = 7, colour="black"), axis.text.x=element_blank(), axis.ticks=element_blank()) + theme(legend.position="bottom") + guides(fill=guide_legend(nrow=8))
```

```{r, echo=FALSE, warning=FALSE}
        ps_df <- phyloseq::psmelt(ps.ng.tax_abund_rel_weighted)
        # 准备数据
        ps_summary <- ps_df %>%
          # 1. 只保留这三个 condition
          filter(pre_post_stroke %in% c("pre.antibiotics", "baseline", "pre.stroke")) %>%

          # 2. 聚合
          group_by(Sex_age, pre_post_stroke, Family) %>%
          summarise(Abundance = sum(Abundance), .groups = "drop") %>%

          # 3. 设置 factor 顺序和重命名
          mutate(
            # 替换 Sex_age 名称
            Sex_age = recode(Sex_age,
                            "female.aged" = "Female (Aged)",
                            "male.aged"   = "Male (Aged)",
                            "male.young"  = "Male (Young)"),
            Sex_age = factor(Sex_age, levels = c("Male (Aged)", "Female (Aged)", "Male (Young)")),

            # 替换 condition 名称
            pre_post_stroke = recode(pre_post_stroke,
                                    "pre.antibiotics" = "Before Antibiotics",
                                    "baseline"        = "Baseline",
                                    "pre.stroke"      = "Before Stroke"),
            pre_post_stroke = factor(pre_post_stroke,
                                    levels = c("Before Antibiotics", "Baseline", "Before Stroke")),

            Family = factor(Family)
          )

        # 确保颜色数匹配
        class_levels <- levels(ps_summary$Family)
        color_map <- setNames(my_colors[seq_along(class_levels)], class_levels)

        # 绘图
        p <- ggplot(ps_summary, aes(x = Sex_age, y = Abundance, fill = Family)) +
          geom_bar(stat = "identity", position = "stack", width = 0.55) +  # 更窄的柱子
          facet_grid(pre_post_stroke ~ ., scales = "free_x", drop = TRUE) +
          scale_fill_manual(values = color_map, drop = FALSE) +
          theme_minimal(base_size = 11) +
          theme(
            axis.text.x = element_text(angle = 45, hjust = 1, size = 9, colour = "black"),
            axis.title = element_text(size = 11),
            strip.text = element_text(size = 10, face = "bold"),
            legend.position = "right",               # ✅ legend 放右边
            legend.title = element_blank(),
            panel.grid.major.x = element_blank(),
            panel.grid.minor = element_blank()
          ) +
          guides(fill = guide_legend(ncol = 2)) +     # 竖排图例
          labs(
            x = "Sex and Age Group",
            y = "Relative Abundance",
            title = "Taxonomic Family Composition by Group and Condition"
          )

        # 保存为 PNG 文件
        ggsave(
          filename = "./figures/Separate_Stroke_and_SexAge_on_Family.png",
          plot = p,
          width = 9,
          height = 6,
          dpi = 200
        )

        knitr::include_graphics("./figures/Separate_Stroke_and_SexAge_on_Family.png")
```


```{r, echo=FALSE, warning=FALSE}
        ps_df <- phyloseq::psmelt(ps.ng.tax_abund_rel_weighted)
        # 数据处理，只保留 "Before Stroke"
        ps_summary <- ps_df %>%
          filter(pre_post_stroke == "pre.stroke") %>%
          group_by(Sex_age, Family) %>%
          summarise(Abundance = sum(Abundance), .groups = "drop") %>%
          mutate(
            Sex_age = recode(Sex_age,
              "female.aged" = "Female (Aged)",
              "male.aged" = "Male (Aged)",
              "male.young" = "Male (Young)"
            ),
            Sex_age = factor(Sex_age, levels = c("Male (Aged)", "Female (Aged)", "Male (Young)")),
            Family = factor(Family)
          )

        # 映射颜色
        class_levels <- levels(ps_summary$Family)
        color_map <- setNames(my_colors[seq_along(class_levels)], class_levels)

        # 绘图
        p <- ggplot(ps_summary, aes(x = Sex_age, y = Abundance, fill = Family)) +
          geom_bar(stat = "identity", position = "stack", width = 0.55) +
          scale_fill_manual(values = color_map, drop = FALSE) +
          theme_minimal(base_size = 11) +
          theme(
            axis.text.x = element_text(angle = 45, hjust = 1, size = 9, colour = "black"),
            axis.title = element_text(size = 11),
            legend.position = "right",
            legend.title = element_blank(),
            panel.grid.major.x = element_blank(),
            panel.grid.minor = element_blank()
          ) +
          labs(
            x = "Sex and Age Group",
            y = "Relative Abundance",
            title = "Family Composition - Before Stroke"
          ) +
          guides(fill = guide_legend(ncol = 2))

        # 保存图像
        ggsave(
          filename = "./figures/Before_Stroke_Family_Composition.png",
          plot = p,
          width = 8,
          height = 5,
          dpi = 200
        )

        # 插入图像到报告
        knitr::include_graphics("./figures/Before_Stroke_Family_Composition.png")
```









\pagebreak

# Alpha diversity
Plot Chao1 richness estimator, Observed OTUs, Shannon index, and Phylogenetic diversity. 
Regroup together samples from the same group.
```{r, echo=FALSE, warning=FALSE}
# using rarefied data
#FITTING2: CONSOLE: 
#gunzip table_even4753.biom.gz
#alpha_diversity.py -i table_even42369.biom --metrics chao1,observed_otus,shannon,PD_whole_tree -o adiv_even.txt -t ../clustering/rep_set.tre
#gunzip table_even4753.biom.gz
#alpha_diversity.py -i table_even4753.biom --metrics chao1,observed_otus,shannon,PD_whole_tree -o adiv_even.txt -t ../clustering_stool/rep_set.tre
#gunzip table_even4753.biom.gz
#alpha_diversity.py -i table_even4753.biom --metrics chao1,observed_otus,shannon,PD_whole_tree -o adiv_even.txt -t ../clustering_swab/rep_set.tre
```


```{r, echo=TRUE, warning=FALSE}
#fig.width=9, fig.height=6,
#QIIME1 hmp.div_qiime <- read.csv("adiv_even.txt", sep="\t")
#QIIME1 colnames(hmp.div_qiime) <- c("sam_name", "chao1", "observed_otus", "shannon", "PD_whole_tree")
#QIIME1 row.names(hmp.div_qiime) <- hmp.div_qiime$sam_name
#QIIME1 div.df <- merge(hmp.div_qiime, hmp.meta, by = "sam_name")
#QIIME1 div.df2 <- div.df[, c("Group", "chao1", "shannon", "observed_otus", "PD_whole_tree")]
#QIIME1 colnames(div.df2) <- c("Group", "Chao-1", "Shannon", "OTU", "Phylogenetic Diversity")
#QIIME1 options(max.print=999999)
#QIIME1 #27     H47 830.5000 5.008482 319               10.60177
#QIIME1 #FITTING4: if occuring "Computation failed in `stat_signif()`:not enough 'y' observations"
#QIIME1 #means: the patient H47 contains only one sample, it should be removed for the statistical p-values calculations.
#QIIME1 #delete H47(1)
#QIIME1 #div.df2 <- div.df2[-c(3), ]
#QIIME1 #div.df2 <- div.df2[-c(55,54, 45,40,39,27,26,25,1), ]


# for QIIME2: Lesen der Metriken
shannon <- read.table("exported_alpha/shannon/alpha-diversity.tsv", header=TRUE, sep="\t")
faith_pd <- read.table("exported_alpha/faith_pd/alpha-diversity.tsv", header=TRUE, sep="\t")
observed <- read.table("exported_alpha/observed_features/alpha-diversity.tsv", header=TRUE, sep="\t")
#chao1 <- read.table("exported_alpha/chao1/alpha-diversity.tsv", header=TRUE, sep="\t")    #TODO: Check the correctness of chao1-calculation.

# Umbenennen für Klarheit
colnames(shannon) <- c("sam_name", "shannon")
colnames(faith_pd) <- c("sam_name", "PD_whole_tree")
colnames(observed) <- c("sam_name", "observed_otus")
#colnames(chao1) <- c("sam_name", "chao1")

# Merge alles in ein DataFrame
div.df <- Reduce(function(x, y) merge(x, y, by="sam_name"),
                  list(shannon, faith_pd, observed))

# Meta-Daten einfügen
div.df <- merge(div.df, hmp.meta, by="sam_name")

# Reformat
div.df2 <- div.df[, c("sam_name", "Group", "shannon", "observed_otus", "PD_whole_tree")]
colnames(div.df2) <- c("Sample name", "Group", "Shannon", "OTU", "Phylogenetic Diversity")
write.csv(div.df2, file="alpha_diversities.txt")
knitr::kable(div.df2) %>% kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))

#https://uc-r.github.io/t_test
#We can perform the test with t.test and transform our data and we can also perform the nonparametric test with the wilcox.test function.
stat.test.Shannon <- compare_means(
 Shannon ~ Group, data = div.df2,
 method = "t.test"
)
knitr::kable(stat.test.Shannon) %>% kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))

div_df_melt <- reshape2::melt(div.df2)
#head(div_df_melt)

#https://plot.ly/r/box-plots/#horizontal-boxplot
#http://www.sthda.com/english/wiki/print.php?id=177
#https://rpkgs.datanovia.com/ggpubr/reference/as_ggplot.html
#http://www.sthda.com/english/articles/24-ggpubr-publication-ready-plots/82-ggplot2-easy-way-to-change-graphical-parameters/
#https://plot.ly/r/box-plots/#horizontal-boxplot
#library("gridExtra")
#par(mfrow=c(4,1))
p <- ggboxplot(div_df_melt, x = "Group", y = "value",
              facet.by = "variable", 
              scales = "free",
              width = 0.5,
              fill = "gray", legend= "right")
#ggpar(p, xlab = FALSE, ylab = FALSE)
lev <- levels(factor(div_df_melt$Group)) # get the variables
#FITTING4: delete H47(1) in lev
#lev <- lev[-c(3)]
# make a pairwise list that we want to compare.
#my_stat_compare_means
#https://stackoverflow.com/questions/47839988/indicating-significance-with-ggplot2-in-a-boxplot-with-multiple-groups
L.pairs <- combn(seq_along(lev), 2, simplify = FALSE, FUN = function(i) lev[i]) #%>% filter(p.signif != "ns")
my_stat_compare_means  <- function (mapping = NULL, data = NULL, method = NULL, paired = FALSE, 
    method.args = list(), ref.group = NULL, comparisons = NULL, 
    hide.ns = FALSE, label.sep = ", ", label = NULL, label.x.npc = "left", 
    label.y.npc = "top", label.x = NULL, label.y = NULL, tip.length = 0.03, 
    symnum.args = list(), geom = "text", position = "identity", 
    na.rm = FALSE, show.legend = NA, inherit.aes = TRUE, ...) 
{
    if (!is.null(comparisons)) {
        method.info <- ggpubr:::.method_info(method)
        method <- method.info$method
        method.args <- ggpubr:::.add_item(method.args, paired = paired)
        if (method == "wilcox.test") 
            method.args$exact <- FALSE
        pms <- list(...)
        size <- ifelse(is.null(pms$size), 0.3, pms$size)
        color <- ifelse(is.null(pms$color), "black", pms$color)
        map_signif_level <- FALSE
        if (is.null(label)) 
            label <- "p.format"
        if (ggpubr:::.is_p.signif_in_mapping(mapping) | (label %in% "p.signif")) {
            if (ggpubr:::.is_empty(symnum.args)) {
                map_signif_level <- c(`****` = 1e-04, `***` = 0.001, 
                  `**` = 0.01, `*` = 0.05, ns = 1)
            } else {
               map_signif_level <- symnum.args
            } 
            if (hide.ns) 
                names(map_signif_level)[5] <- " "
        }
        step_increase <- ifelse(is.null(label.y), 0.12, 0)
        ggsignif::geom_signif(comparisons = comparisons, y_position = label.y, 
            test = method, test.args = method.args, step_increase = step_increase, 
            size = size, color = color, map_signif_level = map_signif_level, 
            tip_length = tip.length, data = data)
    } else {
        mapping <- ggpubr:::.update_mapping(mapping, label)
        layer(stat = StatCompareMeans, data = data, mapping = mapping, 
            geom = geom, position = position, show.legend = show.legend, 
            inherit.aes = inherit.aes, params = list(label.x.npc = label.x.npc, 
                label.y.npc = label.y.npc, label.x = label.x, 
                label.y = label.y, label.sep = label.sep, method = method, 
                method.args = method.args, paired = paired, ref.group = ref.group, 
                symnum.args = symnum.args, hide.ns = hide.ns, 
                na.rm = na.rm, ...))
    }
}

# Rotate the x-axis labels to 45 degrees and adjust their position
p <- p + theme(axis.text.x = element_text(angle = 45, hjust = 1, vjust=1, size=8))
#comparisons = list(c("Group1", "Group2"), c("Group3", "Group4")),
p2 <- p + 
stat_compare_means(
  method="t.test",
  comparisons = list(),
  label = "p.signif",
  symnum.args <- list(cutpoints = c(0, 0.0001, 0.001, 0.01, 0.05, 1), symbols = c("****", "***", "**", "*", "ns"))
)
#comparisons = L.pairs,
#symnum.args <- list(cutpoints = c(0, 0.0001, 0.001, 0.01, 0.05), symbols = c("****", "***", "**", "*")),
#stat_pvalue_manual
print(p2)
#https://stackoverflow.com/questions/20500706/saving-multiple-ggplots-from-ls-into-one-and-separate-files-in-r
#FITTING3: mkdir figures
ggsave("./figures/alpha_diversity_Group.png", device="png", height = 10, width = 15)
ggsave("./figures/alpha_diversity_Group.svg", device="svg", height = 10, width = 15)

#NOTE: Run this Phyloseq.Rmd, then run the code of MicrobiotaProcess.R to manually generate PCoA.png, then run this Phyloseq.Rmd!
#NOTE: AT_FIRST_DEACTIVATE_THIS_LINE: knitr::include_graphics("./figures/PCoA.png")

```



```{r, echo=FALSE, warning=FALSE, fig.cap="Alpha diversity", out.width = '100%', fig.align= "center"}
## MANUALLY selected alpha diversities unter host-env after 'cp alpha_diversities.txt selected_alpha_diversities.txt'
#knitr::include_graphics("./figures/alpha_diversity_Group.png")
#selected_alpha_diversities<-read.csv("selected_alpha_diversities.txt",sep="\t")
#knitr::kable(selected_alpha_diversities) %>% kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))
```



# Beta diversity (Bray-Curtis distance)

## Group1 vs Group2
```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#fig.cap="Beta diversity",

#for QIIME1: file:///home/jhuang/DATA/Data_Marius_16S/core_diversity_e42369/bdiv_even42369_Group/weighted_unifrac_boxplots/Group_Stats.txt

# -- for QIIME2: MANUALLY filter permanova-pairwise.csv and save as permanova-pairwise_.csv
# #grep "Permutations" exported_beta_group/permanova-pairwise.csv > permanova-pairwise_.csv
# #grep "Group1,Group2" exported_beta_group/permanova-pairwise.csv >> permanova-pairwise_.csv
# #grep "Group3,Group4" exported_beta_group/permanova-pairwise.csv >> permanova-pairwise_.csv
# beta_diversity_group_stats<-read.csv("permanova-pairwise_.csv",sep=",")
# #beta_diversity_group_stats <- beta_diversity_group_stats[beta_diversity_group_stats$Group.1 == "Group1" & beta_diversity_group_stats$Group.2 == "Group2", ]
# #beta_diversity_group_stats <- beta_diversity_group_stats[beta_diversity_group_stats$Group.1 == "Group3" & beta_diversity_group_stats$Group.2 == "Group4", ]
# knitr::kable(beta_diversity_group_stats) %>% kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))

#NOTE: Run this Phyloseq.Rmd, then run the code of MicrobiotaProcess.R to manually generate Comparison_of_Bray_Distances_Group1_vs_Group2.png and Comparison_of_Bray_Distances_Group3_vs_Group4.png, then run this Phyloseq.Rmd!

#knitr::include_graphics("./figures/Comparison_of_Bray_Distances_Group1_vs_Group2.png")

```

## Group3 vs Group4
```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#knitr::include_graphics("./figures/Comparison_of_Bray_Distances_Group3_vs_Group4.png")
```





# The PCoA analysis

## Group1 vs Group2
```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#knitr::include_graphics("./figures/PCoA2_Group1_vs_Group2.png")
```

## Group3 vs Group4
```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#knitr::include_graphics("./figures/PCoA2_Group3_vs_Group4.png")
```

## Groups 1, 2, 3 and 4
```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#knitr::include_graphics("./figures/PCoA2_Group1-Group4.png")
```


## Groups 9,10, 11, 12,13, and 14
```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#knitr::include_graphics("./figures/PCoA2_Group9-Group14.png")
```






# Differential abundance analysis

Differential abundance analysis aims to find the differences in the abundance of each taxa between two groups of samples, assigning a significance value to each comparison.

## Group1 vs Group2

```{r, echo=TRUE, warning=FALSE}
#ps.ng.tax [ 2633 taxa and 136 samples] and ps.ng.tax_abund (absolute abundance)  [382 taxa and 136 samples],  ps.ng.tax_abund_rel (relative abundance)  [382 taxa and 136 samples], either ps.ng.tax and ps.ng.tax_abund can be used here!
ps.ng.tax_abund_sel1 <- data.table::copy(ps.ng.tax_abund)
otu_table(ps.ng.tax_abund_sel1) <- otu_table(ps.ng.tax_abund)[,c("sample-A1","sample-A2","sample-A3","sample-A4","sample-A5","sample-A6","sample-A7","sample-A8","sample-A9","sample-A10","sample-A11",   "sample-B1","sample-B2","sample-B3","sample-B4","sample-B5","sample-B6","sample-B7","sample-B8","sample-B9","sample-B10","sample-B11","sample-B12","sample-B13","sample-B14","sample-B15","sample-B16")]
diagdds = phyloseq_to_deseq2(ps.ng.tax_abund_sel1, ~Group)
diagdds$Group <- relevel(diagdds$Group, "Group2")
diagdds = DESeq(diagdds, test="Wald", fitType="parametric")
resultsNames(diagdds)

res = results(diagdds, cooksCutoff = FALSE)
alpha = 0.05
sigtab = res[which(res$padj < alpha), ]
sigtab = cbind(as(sigtab, "data.frame"), as(phyloseq::tax_table(ps.ng.tax_abund_sel1)[rownames(sigtab), ], "matrix"))
#sigtab <- sigtab[rownames(sigtab) %in% rownames(phyloseq::tax_table(ps.ng.tax_abund)), ]
kable(sigtab) %>%
  kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))

library("ggplot2")
theme_set(theme_bw())
scale_fill_discrete <- function(palname = "Set1", ...) {
    scale_fill_brewer(palette = palname, ...)
}
x = tapply(sigtab$log2FoldChange, sigtab$Order, function(x) max(x))
x = sort(x)
sigtab$Order = factor(as.character(sigtab$Order), levels=names(x))
x = tapply(sigtab$log2FoldChange, sigtab$Family, function(x) max(x))
x = sort(x)
sigtab$Family = factor(as.character(sigtab$Family), levels=names(x))
ggplot(sigtab, aes(x=log2FoldChange, y=Family, color=Order)) + geom_point(aes(size=padj)) + scale_size_continuous(name="padj",range=c(8,4))+
  theme(axis.text.x = element_text(angle = -25, hjust = 0, vjust=0.5))
```

```{r, echo=FALSE, warning=FALSE, out.width = '100%', fig.align= "center"}
#knitr::include_graphics("./figures/diff_analysis_Group1_vs_Group2.png")
```

## Group3 vs Group4

```{r, echo=TRUE, warning=FALSE}
ps.ng.tax_abund_sel2 <- data.table::copy(ps.ng.tax_abund)
otu_table(ps.ng.tax_abund_sel2) <- otu_table(ps.ng.tax_abund)[,c("sample-C1","sample-C2","sample-C3","sample-C4","sample-C5","sample-C6","sample-C7","sample-C8","sample-C9","sample-C10",   "sample-E1","sample-E2","sample-E3","sample-E4","sample-E5","sample-E6","sample-E7","sample-E8","sample-E9","sample-E10")]
diagdds = phyloseq_to_deseq2(ps.ng.tax_abund_sel2, ~Group)
diagdds$Group <- relevel(diagdds$Group, "Group4")
diagdds = DESeq(diagdds, test="Wald", fitType="parametric")
resultsNames(diagdds)

res = results(diagdds, cooksCutoff = FALSE)
alpha = 0.05
sigtab = res[which(res$padj < alpha), ]
sigtab = cbind(as(sigtab, "data.frame"), as(phyloseq::tax_table(ps.ng.tax_abund_sel2)[rownames(sigtab), ], "matrix"))

kable(sigtab) %>%
  kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))

library("ggplot2")
theme_set(theme_bw())
scale_fill_discrete <- function(palname = "Set1", ...) {
    scale_fill_brewer(palette = palname, ...)
}
x = tapply(sigtab$log2FoldChange, sigtab$Order, function(x) max(x))
x = sort(x)
sigtab$Order = factor(as.character(sigtab$Order), levels=names(x))
x = tapply(sigtab$log2FoldChange, sigtab$Family, function(x) max(x))
x = sort(x)
sigtab$Family = factor(as.character(sigtab$Family), levels=names(x))
ggplot(sigtab, aes(x=log2FoldChange, y=Family, color=Order)) + geom_point(aes(size=padj)) + scale_size_continuous(name="padj",range=c(8,4))+
  theme(axis.text.x = element_text(angle = -25, hjust = 0, vjust=0.5))
```


```{r, echo=FALSE, warning=FALSE, out.width = '200%', fig.align= "center"}
#knitr::include_graphics("./figures/diff_analysis_Group3_vs_Group4.png")
```






```{r, echo=FALSE, warning=FALSE}
## The table below shows the raw counts of the 199 OTUs across all samples, with OTUs as rows and samples as columns.
#kable(otu_table(ps.ng.tax)) %>%
#kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))
```



```{r, echo=FALSE, warning=FALSE}
## The table below shows the taxonomic assignments of the 199 OTUs, with OTUs as rows and their corresponding taxonomic ranks as columns.
# ~/Tools/csv2xls-0.4/csv_to_xls.py otu_table.csv tax_table.csv -d',' -o otu_tax.xls;
#kable(taxonomy_df) %>%
#  kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))
```



```{r, echo=FALSE, warning=FALSE}
## The sample L9 retained only 413 sequences after the complete preprocessing workflow, which includes filtering, denoising, merging, and chimera removal and was excluded from downstream analyses.
# # Read the TSV file
# ~/Tools/csv2xls-0.4/csv_to_xls.py denoising-stats.csv -d$'\t' -o preprocessing_stats.xls;
# denoising_stats <- read.csv("denoising-stats.csv", sep="\t")
# # Display the table
# kable(denoising_stats, caption = "Preprocessing statistics for each sample") %>%
#   kable_styling(bootstrap_options = c("striped", "hover", "condensed", "responsive"))
```

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