LOCUS       p602_LT      2454 bp    DNA     linear   UNA 23-Feb-2016
DEFINITION  
FEATURES             Location/Qualifiers
     CDS             1..2454
                     /cds_type="ORF"
                     /gene="LT"
                     /codon_start=1
                     /product="large T antigen LT"
                     /protein_id="AEM01097.1"
                     /translation="MDLVLNRKEREALCKLLEIAPNCYGNIPLMKAAFKRSCLKHHPD
                     KGGNPVIMMELNTLWSKFQQNIHKLRSDFSMFDEVDEAPIYGTTKFKEWWRSGGFSFG
                     KAYEYGPNPHGTNSRSRKPSSNASRGAPSGSSPPHSQSSSSGYGSFSASQASDSQSRG
                     PDIPPEHHEEPTSSSGSSSREETTNSGRESSTPNGTSVPRNSSRTDGTWEDLFCDESL
                     SSPEPPSSSEEPEEPPSSRSSPRQPPSSSAEEASSSQFTDEEYRSSSFTTPKTPPPFS
                     RKRKFGGSRSSASSASSASFTSTPPKPKKNRETPVPTDFPIDLSDYLSHAVYSNKTVS
                     CFAIYTTSDKAIELYDKIEKFKVDFKSRHACELGCILLFITLSKHRVSAIKNFCSTFC
                     TISFLICKGVNKMPEMYNNLCKPPYKLLQENKPLLNYEFQEKEKEASCNWNLVAEFAC
                     EYELDDHFIILAHYLDFAKPFPCQKCENRSRLKPHKAHEAHHSNAKLFYESKSQKTIC
                     QQAADTVLAKRRLEMLEMTRTEMLCKKFKKHLERLRDLDTIDLLYYMGGVAWYCCLFE
                     EFEKKLQKIIQLLTENIPKYRNIWFKGPINSGKTSFAAALIDLLEGKALNINCPSDKL
                     PFELGCALDKFMVVFEDVKGQNSLNKDLQPGQGINNLDNLRDHLDGAVAVSLEKKHVN
                     KKHQIFPPCIVTANDYFIPKTLIARFSYTLHFSPKANLRDSLDQNMEIRKRRILQSGT
                     TLLLCLIWCLPDTTFKPCLQEEIKNWKQILQSEISYGKFCQMIENVEAGQDPLLNILI
                     EEEGPEETEETQDSGTFSQ"
                     /label="LT"
     misc_feature    <1..>2454
                     /feature_type="Insertion"
                     /origin="MCPyV LT aus p460 insert (2.494bp
                     linear)Fragment: from NotI cut at 4^5 to NotI cut at
                     2486^2487, 2.482bpOrientation: originalPlus strand 5':
                     no changePlus strand 3':   no changeMinus strand 5':  no
                     changeMinus strand 3':  no change"
                     /label="Insert"
     Region          <1..194
                     /label="Sequenziert"
     Site            346..387
                     /site_type="binding site"
                     /label="CM2B4 epitope"
     Site            730..735
                     /site_type="mutagenized site"
                     /label="Mut nach CGGTGA für MCCL-12 (p448)"
     conflict        1122
                     /label="conflict zu Addgene"
     conflict        1170
                     /label="conflict zu Addgene"
     conflict        1281
                     /label="conflict zu Addgene"
     Region          1431..2454
                     /label="Sequenziert"
     mutation        1489
                     /label="C nach T"
ORIGIN
        1 ATGGATTTAG TCCTAAATAG GAAAGAAAGA GAGGCTCTCT GCAAGCTTTT AGAGATTGCT
       61 CCTAATTGTT ATGGCAACAT CCCTCTGATG AAAGCTGCTT TCAAAAGAAG CTGCTTAAAG
      121 CATCACCCTG ATAAAGGGGG AAATCCTGTT ATAATGATGG AATTGAACAC CCTTTGGAGC
      181 AAATTCCAGC AAAATATCCA CAAGCTCAGA AGTGACTTCT CTATGTTTGA TGAGGTTGAC
      241 GAGGCCCCTA TATATGGGAC CACTAAATTC AAAGAATGGT GGAGATCAGG AGGATTCAGC
      301 TTCGGGAAGG CATACGAATA TGGGCCCAAT CCACACGGGA CCAACTCAAG ATCCAGAAAG
      361 CCTTCCTCCA ATGCATCCAG GGGAGCCCCC AGTGGAAGCT CACCACCCCA CAGCCAGAGC
      421 TCTTCCTCTG GGTATGGGTC CTTCTCAGCG TCCCAGGCTT CAGACTCCCA GTCCAGAGGA
      481 CCCGATATAC CTCCCGAACA CCATGAGGAA CCCACCTCAT CCTCTGGATC CAGTAGCAGA
      541 GAGGAGACCA CCAATTCAGG AAGAGAATCC AGCACACCCA ATGGAACCAG TGTACCTAGA
      601 AATTCTTCCA GAACTGATGG CACCTGGGAG GATCTCTTCT GCGATGAATC ACTTTCCTCC
      661 CCTGAGCCTC CCTCGTCCTC TGAGGAGCCT GAGGAGCCCC CCTCCTCAAG AAGCTCGCCC
      721 CGGCAGCCCC CGTCTTCCTC TGCCGAGGAG GCCTCGTCAT CTCAGTTTAC AGATGAGGAA
      781 TACAGATCCT CCTCCTTCAC CACCCCGAAG ACCCCTCCTC CATTCTCAAG AAAGCGAAAA
      841 TTTGGGGGGT CCCGAAGCTC TGCAAGCTCT GCTAGTTCAG CAAGTTTTAC AAGCACTCCA
      901 CCAAAGCCAA AAAAGAACAG AGAAACTCCT GTTCCTACTG ATTTTCCTAT TGATCTTTCT
      961 GATTATCTTA GCCATGCTGT ATATAGTAAT AAAACTGTTT CATGTTTTGC CATTTATACT
     1021 ACTTCTGATA AAGCTATAGA GTTATATGAT AAGATTGAGA AATTTAAAGT TGATTTTAAA
     1081 AGCAGGCATG CCTGTGAATT AGGATGTATT TTATTGTTTA TcACTTTATC AAAGCATAGA
     1141 GTATCTGCTA TTAAGAATTT TTGCTCTACt TTCTGCACTA TAAGCTTTTT AATTTGTAAA
     1201 GGAGTGAATA AGATGCCTGA AATGTATAAT AATTTATGCA AGCCCCCTTA CAAATTACTG
     1261 CAAGAGAATA AGCCACTGCT cAATTATGAA TTTCAAGAAA AAGAAAAAGA GGCCAGCTGT
     1321 AATTGGAATT TAGTTGCTGA ATTTGCTTGT GAATATGAGC TAGACGACCA CTTTATTATC
     1381 TTAGCCCATT ATCTAGACTT TGCAAAACCA TTTCCTTGCC AAAAGTGTGA AAACAGATCT
     1441 CGCCTCAAAC CTCACAAGGC TCATGAGGCT CATCATTCTA ATGCTAAGTT ATTTTATGAA
     1501 TCTAAATCTC AGAAAACCAT TTGCCAACAA GCCGCAGACA CTGTTCTAGC CAAAAGGAGG
     1561 TTAGAGATGC TGGAAATGAC CAGGACAGAA ATGCTATGTA AGAAGTTTAA GAAGCACCTG
     1621 GAGAGATTAA GAGATTTAGA CACAATAGAT CTACTGTATT ATATGGGTGG TGTGGCCTGG
     1681 TACTGCTGCT TATTTGAAGA GTTTGAAAAG AAGCTGCAGA AAATTATTCA ATTATTAACA
     1741 GAGAATATAC CTAAGTATAG AAACATTTGG TTTAAAGGGC CTATTAACAG TGGAAAAACA
     1801 AGCTTTGCTG CAGCCTTAAT AGATTTGCTA GAAGGGAAGG CCTTGAATAT AAACTGTCCA
     1861 TCTGATAAAC TACCTTTTGA ACTAGGATGT GCTTTGGATA AATTTATGGT TGTTTTTGAG
     1921 GATGTGAAAG GGCAAAATAG CCTAAATAAA GATCTGCAAC CAGGGCAAGG AATAAATAAC
     1981 CTTGATAACT TAAGAGATCA TCTAGATGGT GCTGTAGCTG TAAGCTTAGA GAAGAAGCAT
     2041 GTGAATAAGA AGCATCAGAT TTTTCCTCCT TGTATTGTTA CTGCTAATGA TTATTTTATT
     2101 CCCAAAACAT TAATAGCAAG ATTTAGTTAT ACTTTACACT TTTCCCCAAA AGCGAATCTA
     2161 AGAGATTCCC TGGATCAGAA CATGGAAATA AGAAAAAGAA GAATTCTTCA AAGTGGAACC
     2221 ACTTTATTGC TTTGTCTTAT TTGGTGCTTG CCTGATACAA CCTTTAAGCC TTGCTTACAA
     2281 GAAGAAATTA AAAACTGGAA GCAAATTTTA CAGAGTGAAA TATCATATGG TAAATTTTGT
     2341 CAAATGATAG AAAATGTAGA AGCTGGTCAG GACCCTCTGC TCAATATTCT TATTGAGGAA
     2401 GAGGGCCCTG AGGAAACTGA AGAAACCCAA GATTCTGGTA CTTTTTCTCA ATAA
//
